Dr. Leonie Vollmar
Institute of Physical Chemistry
Albertstraße 21
79104 Freiburg
Phone: +49 761 203-96704
Room: 01.003 1.OG Flachbau
Email: leonie.vollmar@physchem.uni-freiburg.de
About me
- since 11/2019 - 07/2024: PhD student in the Hugel group
- 10/2017 - 09/2019: MSc Biochemistry and Biophysics
University of Freiburg, Germany
Master thesis: Quantitative Comparison of a sCMOS and an EMCCD Camera for Detection of smFRET Dynamics - 09/2016 - 07/2017: MSc Interdiciplinary Approaches in Life Sciences M1
Centre des Recherches Interdisciplinaires Paris (CRI), Université Paris Descartes, France - 10/2013 - 08/2016: BSc Wirtschaftschemie (chemistry and management)
Ulm University, Germany
Research
Single-molecule FRET to investigate the Hsp90 machinery on a multi-colour TIRF-setup
Hsp90 is one of the most abundant proteins in eukaryotic cells. It acts as a chaperone for kinases and therefore interacts with many different co-chaperones.
The question I want to address is how these complexes form. Do these proteins just randomly bump into each other or is there a certain directionality and sequence of binding events? Who recruits whom? What are their dynamics?
Funded by the SFB 1381
Shared first author publications
Vollmar L, Bebon R, Schimpf J, Flietel B, Celiksoy S, Soennichsen C, Godec A and Hugel T. (2024) Model-free inference of memory in conformational dynamics of a multi-domain protein J. Phys. A: Math. Theor.
Vollmar L, Schimpf J, Hermann B, Hugel T. (2024) Cochaperones convey the energy of ATP hydrolysis for directional action of Hsp90 Nat. Commun.
Mondol T, Silbermann LM, Schimpf J, Vollmar L, Hermann B, Tych KK, Hugel T. (2023) Aha1 regulates Hsp90's conformation and function in a stoichiometry-dependent way. Biophys J.
Other collaborative publications
Tancredi R, Feltrin A, Infirri GS, Toso S, Vollmar L, Hugel T, Baiesi M. (2024) Constrained hidden Markov models reveal further Hsp90 protein states New J. Phys.
Götz M; Barth A; Bohr S; Börner R; Chen J; Cordes T; Erie D; Gebhardt C; Hadzic M; Hamilton G; Hatzakis N; Hugel T; Kisley L; Lamb D; de Lannoy C; Mahn C; Dunukara D; de Ridder D; Sanabria H; Schimpf J, Seidel C, Sigel R, Sletfjerding M, Thomsen J, Vollmar L, Wanninger S, Weninger K, Xu P, Schmid S (2022) A blind benchmark of analysis tools to infer kinetic rate constants from single-molecule FRET trajectories. Nat. Commun.
J. Nuno de Sousa Machado, Leonie Vollmar, Julia Schimpf, Paushali Chaudhury, Rashmi Kumariya, Chris van der Does, Thorsten Hugel, Sonja-Verena Albers
Autophosphorylation of the KaiC-like protein ArlH inhibits oligomerisation and interaction with ArlI, the motor ATPase of the archaellum Mol Microbiol (2021)