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Selected publications

JoVE Video: Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

JoVE Video: Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

JoVE Video: Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy



Steffen Wolf, Benedikt Sohmen, Björn Hellenkamp, Johann Thurn, Gerhard Stock and Thorsten Hugel 
Hierarchical dynamics in allostery following ATP hydrolysis monitored by single molecule FRET measurements and MD simulations
Chem.Sci. (2021);


M. Tschaikowsky, M. Selig, S. Brander, B.N. Balzer, T. Hugel & B. Rolauffs
Proof-of-concept for the detection of early osteoarthritis pathology by clinically applicable endomicroscopy and quantitative AI-supported optical biopsy
Osteoarthritis and Cartilage, S1063-4584, 20, 31167-5 (2020);

H. Wang, M. Lallemang, B. Hermann, C. Wallin, R. Loch, A. Blanc, B.N. Balzer, T. Hugel & J. Luo
ATP impedes the inhibitory effect of Hsp90 on Aβ40 fibrillation
J. Mol. Biol., S0022-2836, 20, 30635-5 (2020);

S. Schmid & T. Hugel
Controlling protein function by fine-tuning conformational flexibility
eLife, 9, 1013 (2020);

S. Wolf, B. Sohmen, B. Hellenkamp, J. Thurn, G. Stock & T. Hugel
Allosteric action of nucleotides on Hsp90 across several time- and length scales
bioRxiv, 2020.02.15.950725 (2020);

A. Kolberg, C. Wenzel, T. Hugel, M. Gallei & B.N. Balzer
Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
J. Vis. Exp., 157, e60934 (2020).

M. Marczynski, B.N. Balzer, K. Jiang, T. M. Lutz, T. Crouzier & O. Lieleg
Charged Glycan Residues Critically Contribute to the Adsorption and Lubricity of Mucins

Colloids and Surfaces B: Biointerfaces, 187, 110614 (2020).


M. Schmid & T. Hugel
Same Equilibrium. Different Kinetics. Protein Functional Consequences
bioRxiv, 838938 (2019).

M. Tschaikowsky, S. Brander, B.N. Balzer, B. Rolauffs & T. Hugel
Proof-of-concept for the detection of early osteoarthritis pathology by endomicroscopy
bioRxiv, 734368 (2019).

A. Kolberg, C.Wenzel, K. Hackenstrass, R. Schwarzl, C. Rüttiger, T. Hugel, M. Gallei, R.R. Netz & B.N. Balzer
Opposing temperature dependence of the stretching response of single PEG and PNiPAM polymers
J. Am. Chem. Soc., 141 (29), 11603 (2019).

H. Schafer, A. Kolberg, M. Gockeln, R. Kun, B.N. Balzer & K. Koschek
Influence of free PCL in PCL/PBA-a copolymers and blends on morphology, thermo-mechanical and shape memory properties
Polymer Testing, 77, 105888 (2019).

S. Brander, T. Jank & T.Hugel
AFM Imaging Suggests Receptor-Free Penetration of Lipid Bilayers by Toxins
Langmuir, 35 (2), 365 (2019).


B. Hellenkamp, J. Thurn, M. Stadlmeier & T.Hugel
Kinetics of Transient Protein Complexes Determined via Diffusion-Independent Microfluidic Mixing and Fluorescence Stoichiometry
J. Phys. Chem. B, 122 (49), 11554 (2018).

K.M. Tych, M. Jahn, F. Gegenfurtner, V.K. Hechtl, J. Buchner, T. Hugel & M. Rief
Nucleotide-Dependent Dimer Association and Dissociation of the Chaperone Hsp90
J. Phys. Chem. B, 122 (49), 11373 (2018).

B. Hellenkamp, S. Schmid, et int. & T. Hugel
Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
Nat Methods,15, 669 (2018).
Open access:

W. Ye, M. Götz, S. Celiksoy, L. Tüting, C. Ratzke, J. Prasad, J. Ricken, S.V. Wegner, R. Ahijado-Guzmán, T. Hugel & C. Sönnichsen
Conformational Dynamics of a Single Protein Monitored for 24 h at Video Rate
Nano Lett., 18 (10), 6633 (2018).
Open access:

M. Jahn, K. Tych, H. Girstmair, M. Steinmaßl, T. Hugel, J. Buchner & M. Rief
Folding and Domain Interactions of Three Orthologs of Hsp90 Studied by Single-Molecule Force Spectroscopy
Structure 26 (1), 96 (2018).

S. Schmid, M. Götz & T. Hugel
Effects of inhibitors on Hsp90's conformational dynamics, cochaperone and client interactions
ChemPhysChem19 (14), 1716 (2018).

S. Schmid & T. Hugel
Efficient use of single molecule time traces to resolve kinetic rates, models and uncertainties
J. Chem. Phys., 148, 123312 (2018).

M. Götz, P. Wortmann, S. Schmid & T. Hugel
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
J. Vis. Exp. 131, e56896 (2018).


P. Wortmann, M. Götz & T. Hugel
Cooperative Nucleotide Binding in Hsp90 and Its Regulation by Aha1
Biophys J., 113 (8), 1711 (2017).

B. Winkeljann, K. Boettcher, B.N. Balzer & O. Lieleg
Mucin Coatings Prevent Tissue Damage at the Cornea–Contact Lens Interface
Adv. Mater. Interfaces, 4 (19), 1700186 (2017).

S. Liese, M. Gensler, S. Krysiak, R. Schwarzl, A. Achazi, B. Paulus, T. Hugel, J. Rabe & R. Netz
Hydration Effects Turn a Highly Stretched Polymer from an Entropic into an Energetic Spring
ACS Nano, 11 (1), 702 (2017).

B. Hellenkamp, P. Wortmann, F. Kandzia, M. Zacharias & T. Hugel
Multidomain structure and correlated dynamics determined by self-consistent FRET networks
Nat Methods, 14 (2), 174 (2017).


K. Boettcher, S. Kienle, J. Nachtsheim, R. Burgkart, T. Hugel & O. Lieleg
The structure and mechanical properties of articular cartilage are highly resilient towards transient dehydration
Acta Biomaterialia, 29, 180 (2016).

M. Götz, P. Wortmann, S. Schmid & T. Hugel
A Multicolor Single-Molecule FRET Approach to Study Protein Dynamics and Interactions Simultaneously
Methods Enzymol., 581, 487 (2016).

S. Schmid, M. Götz & T. Hugel
Single-Molecule Analysis beyond Dwell Times: Demonstration and Assessment in and out of Equilibrium
Biophysical J., 111 (7), 1375 (2016).

F. Stetter, S-H. Hyun, S. Brander, J. Urban, D. Thompson & T. Hugel
Nanomechanical characterization of lipid bilayers with AFM-based methods
Polymer, 102, 326 (2016).

N. Schwierz, S. Krysiak, T. Hugel & M. Zacharias
Mechanism of Reversible Peptide-Bilayer Attachment: Combined Simulation and Experimental Single-Molecule Study
Langmuir, 32 (3), 810 (2016).

M. Jahn, J. Buchner, T. Hugel & M. Rief
Folding and assembly of the large molecular machine Hsp90 studied in single-molecule experiments
PNAS, 113 (5), 1232 (2016).

B.N. Balzer & T. Hugel
Single-Molecule Detection and Manipulation.
In: Saleem Hashmi (editor-in-chief); Reference Module in Materials Science and Materials Engineering. Oxford: Elsevier pp. 1-19 (2016)


B. Kracke, J. Cole, C. Kaiser, B. Hellenkamp, S. Krysiak, A. Ghoorchian, G. Braun, N. Holland & T. Hugel
Thermoswitchable Nanoparticles Based on Elastin-like Polypeptides
Macromolecules, 48 (16), 5868 (2015).

S. Kienle, K. Boettcher, L. Wiegleb, J. Urban, R. Burgkart, O. Lieleg & T. Hugel
Comparison of friction and wear of articular cartilage on different length scales
J. Biomech., 48 (12), 3052 (2015).

S. Krysiak, Q. Wei, K. Rischka, A. Hartwig, R. Haag & T. Hugel
Adsorption mechanism and valency of catechol-functionalized hyperbranched polyglycerols
Beilstein J. Org. Chem., 11, 828 (2015).

F. Stetter, S. Kienle, S. Krysiak & T. Hugel
Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
J. Vis. Exp., 96, e52456 (2015).


C. Ratzke, B. Hellenkamp & T. Hugel
Four-colour FRET reveals directionality in the Hsp90 multicomponent machinery
Nat Commun., 5, 4192 (2014).
Research Highlight:
Four-color FRET follows Hsp90 states
Nat Methods, 11, 791 (2014).

M. Jahn, A. Rehn, B. Pelz, B. Hellenkamp, K. Richter, M. Rief, J. Buchner & T. Hugel
The charged linker of the molecular chaperone Hsp90 modulates domain contacts and biological function
PNAS, 111 (50), 17881 (2014).

S. Schwenkert, T. Hugel & M. B. Cox
The Hsp90 ensemble: coordinated Hsp90–cochaperone complexes regulate diverse cellular processes
Nat Struct Mol Biol, 21 (12), 1021 (2014).

S. Krysiak, S. Liese, R.R. Netz & T. Hugel
Peptide Desorption Kinetics from Single Molecule Force Spectroscopy Studies
J. Am. Chem. Soc., 136 (2), 688 (2014).

B.N. Balzer, S. Kienle, M. Gallei, R. v. Klitzing, M. Rehahn & T. Hugel
Stick-Slip Mechanisms at the Nanoscale
Soft Materials, 12 (1), 106 (2014).

Q. Wei, S. Krysiak, K. Achazi, T. Becherer, P.-L.M. Noeske, F. Paulus, H. Liebe, I. Grunwald, J. Dernedde, A. Hartwig, T. Hugel & R. Haag
Multivalent anchored and crosslinked hyperbranched polyglycerol monolayers as antifouling coating for titanium oxide surfaces
Colloids and Surfaces B: Biointerfaces, 122, 684 (2014).

F.W.S. Stetter, L. Cwiklik, P. Jungwirth & T. Hugel
Single Lipid Extraction: The Anchoring Strength of Cholesterol in Liquid-Ordered and Liquid-Disordered Phases
Biophys J., 107 (5), 1167 (2014).

S. Kienle, M. Gallei, H. Yu, B. Zhang, S. Krysiak, B.N. Balzer, M. Rehahn, A.D. Schlüter & T. Hugel
Effect of Molecular Architecture on Single Polymer Adhesion
Langmuir, 30 (15), 4351 (2014).

C. Lumme, et al.
Nucleotides and Substrates Trigger the Dynamics of the Toc34 GTPase Homodimer Involved in Chloroplast Preprotein Translocation
Structure, 22 (4), 526 (2014).

J. Elbert, F. Krohm, C. Rüttiger, S. Kienle, H. Didzoleit, B.N. Balzer, T. Hugel, B. Stühn, M. Gallei & A. Brunsen
Polymer-Modified Mesoporous Silica Thin Films for Redox-Mediated Selective Membrane Gating
Adv. Funct. Mater., 24 (11), 1591 (2014).

S. Hertrich, F.W.S. Stetter, A. Rühm, T. Hugel & B. Nickel
Highly Hydrated Deformable Polyethylene Glycol-Tethered Lipid Bilayers
Langmuir, 30 (31), 9442 (2014).


B.N. Balzer, M. Gallei, K. Sondergeld, M. Schindler, P. Müller-Buschbaum, M. Rehahn & T. Hugel
Cohesion Mechanisms of Polystyrene-Based Thin Polymer Films
Macromolecules, 46 (18), 7406 (2013).

B.N. Balzer, S. Micciulla, S. Dodoo, M. Zerball, M. Gallei, M Rehahn, R. v. Klitzing & T. Hugel
Adhesion Property Profiles of Supported Thin Polymer Films
ACS Applied Materials & Interfaces, 5 (13), 6300 (2013).

B.N. Balzer, M. Gallei, M. Hauf, M. Stallhofer, L. Wiegleb, A. Holleitner, M. Rehahn & T. Hugel
Nanoscale Friction Mechanisms at Solid–Liquid Interfaces
Angew. Chem. Int. Ed., 52 (25), 6541 (2013).

B.N. Balzer, M. Gallei, M. Hauf, M. Stallhofer, L. Wiegleb, A. Holleitner, M. Rehahn & T. Hugel
Reibungsmechanismen auf der Nanoskala an Fest‐flüssig‐Grenzflächen
Angew. Chem., 2013, 125, 6670 (2013).

M. Khaneft, B. Stühn, J. Engstler, H. Tempel, J. Schneider, T. Pirzer & T. Hugel
Imbibition of polystyrene melts in aligned carbon nanotube arrays
J. Appl. Phys., 113, 074305 (2013).

S. Kienle, T. Pirzer, S. Krysiak, M. Geisler & T. Hugel
Measuring the interaction between ions, biopolymers and interfaces – one polymer at a time
Faraday Discuss.,160, 329 (2013).

F.W.S. Stetter & T. Hugel
The nanomechanical properties of Lipid Membranes are significantly influenced by the presence of Ethanol
Biophys J., 104 (5), 1049 (2013).

S. Dodoo, B.N. Balzer, T. Hugel, A. Laschewsky & R. v. Klitzing
Effect of ionic strength and layer number on swelling of polyelectrolyte multilayers in water vapour
Soft Materials, 11(2), 157 (2013).


N. Schwierz, D. Horinek, S. Liese, T. Pirzer, B.N. Balzer, T. Hugel & R.R. Netz
On the relationship between peptide adsorption resistance and surface contact angle: A combined experimental and simulation single-molecule study
J. Am. Chem. Soc., 134 (48), 19628 (2012).

C. Ratzke, M. N. T. Nguyen, M. P. Mayer & T. Hugel
From a Ratchet Mechanism to Random Fluctuations Evolution of Hsp90's Mechanochemical Cycle
J Mol Biol., 423 (3), 462 (2012).

S. Kienle, S. Liese, N. Schwierz, R.R. Netz & T.Hugel
The effect of temperature on single polypeptide adsorption
ChemPhysChem, 13 (4), 982 (2012).

C. Ratzke, F. Berkemeier & T. Hugel
Hsp90's mechano-chemical cycle is dominated by thermal fluctuations
PNAS, 109 (1), 161 (2012).

B.N. Balzer & T. Hugel
Surface & Interface Characterization: Single Molecule Detection & Manipulation
Polymer Science: A Comprehensive Reference; Elsevier: Amsterdam; pp. 629-645 (2012)


D. Staple, M. Geisler, T. Hugel, L. Kreplak & H. Kreuzer
Forced desorption of polymers from interfaces
New J. Phys., 13, 013025 (2011).

S. Schmid & T. Hugel
Regulatory Posttranslational Modifications in Hsp90 Can Be Compensated by Cochaperone Aha1
Mol Cell., 41 (6), 619 (2011).


M. Geisler, S.B. Xiao, E.M. Puchner, F. Gräter & T. Hugel
Controlling the Structure of Proteins at Surfaces
J. Am. Chem. Soc., 132 (48), 17277 (2010).

C. Ratzke, M. Mickler, B. Hellenkamp, J. Buchner & T. Hugel
The dynamics of Hsp90 C-terminal dimerization is an important part of its conformational cycle
PNAS, 107 (37), 16101 (2010).

T. Hugel & C. Lumme
Bio-inspired novel design principles for artificial molecular motors
Curr. Opin. Biotechnol., 21 (5), 683 (2010).

I. Amin, M. Steenackers, N. Zhang, A. Beyer, X. Zhang, T. Pirzer, T. Hugel, R. Jordan & A. Gölzhäuser
Polymer Carpets
Small, 6 (15), 1623 (2010).

M. Geisler & T. Hugel
Aging of Hydrogenated and Oxidized Diamond
Adv. Mater., 22 (3), 398 (2010).

M. Geisler, R.R. Netz & T. Hugel
Pulling a single molecule off a substrate reveals binding thermodynamics of cosolutes
Angew. Chem. Int. Ed., 49 (28), 4730 (2010).

M. Geisler, R.R. Netz & T. Hugel
Abziehen eines einzelnen Polymers von einem Substrat erschließt thermodynamische Parameter der Kosolutbindung
Angew. Chem., 122 (28), 4838 (2010).


M. Geisler, B.N. Balzer & T. Hugel
Polymer Adhesion at the Solid–Liquid Interface Probed by a Single–Molecule Force Sensor
Small, 5 (24), 2864 (2009).

M. Geisler, D. Horinek & T. Hugel
Single Molecule Adhesion Mechanics on Rough Surfaces
Macromolecules, 42 (23), 9338 (2009).

T. Pirzer & T. Hugel
Adsorption Mechanism of Polypeptides and Their Location at Hydrophobic Interfaces
ChemPhysChem, 10 (16), 2795 (2009).

T. Pirzer & T. Hugel
AFM spring constant determination in viscous liquids
Rev. Sci. Instrum., 80 (3), 035110 (2009).

T. Pirzer, M. Geisler, T. Scheibel & T. Hugel
Single molecule force measurements delineate salt, pH and surface effects on Biopolymer Adhesion
Phys. Biol., 6 (2), 025004 (2009).

M. Mickler, M. Hessling, C. Ratzke, J. Buchner & T. Hugel
The large conformational changes of Hsp90 are only weakly coupled to ATP-hydrolysis
Nat Struct Mol Biol, 16, 281 (2009).


M. Mickler, E. Schleiff & T. Hugel
From biological towards artificial molecular motors
ChemPhysChem, 9 (11), 1503 (2008).

D. Horinek, A. Serr, M. Geisler, T. Pirzer, U. Slotta, S.Q. Lud, J.A. Garrido, T. Scheibel, T. Hugel & R.R. Netz
Peptide adsorption on a hydrophobic surface results from an interplay of solvation, surface, and intrapeptide forces
PNAS, 105 (8), 2842 (2008).

M. Geisler, T. Pirzer, C. Ackerschott, S. Lud, J. Garrido, T. Scheibel & T. Hugel
Influence of Hofmeister Salts on the adhesion of spider silk proteins onto hydrophobic substrates: an AFM-based single molecule study
Langmuir, 24 (4), 1350 (2008).

M. Mickler & T. Hugel
Von molekularen Motoren zu künstlichen Nanomaschinen
Physik in unserer Zeit, 39 (1), 14 (2008).


T. Hugel, J. Michaelis, C. Hetherington, P.J. Jardine, S. Grimes, J.M. Walter, W. Falk, D.L. Anderson & C. Bustamante
Experimental Test of Connector Rotation during DNA Packaging into Bacteriophage phi29 Capsids
PLoS Biol, 5(3): e59 (2007).


G. Neuert, T. Hugel, R.R. Netz & H.E. Gaub
Elasticity of Poly(azobenzene-peptides)
Macromolecules, 39 (2), 789 (2006).


R. Lugmaier, T. Hugel, M. Benoit & H.E. Gaub
Phase contrast and DIC illumination for AFM hybrids
Ultramicroscopy, 104 (3-4), 255 (2005).

T. Hugel, M. Rief, H.E. Gaub & R.R. Netz
Enthalpic Elasticity of Highly Stretched Single Polymers: Quantitative Comparison of Ab-Initio Theory and AFM Experiments
Phys. Rev. Lett., 94, 048301 (2005).


M. Seitz, C. Friedsam, W. Jöstl, T. Huge & H.E. Gaub
Probing Surfaces with Single Polymers
ChemPhysChem, 4 (9), 986 (2003).

N.B. Holland, T. Hugel, G. Neuert, D. Oesterhelt, L. Moroder, M. Seitz & H.E. Gaub
Single Molecule Force Spectroscopy of Azobenzene Polymers: Switching Elasticity of Single Photochromic Macromolecules
Macromolecules, 36 (6), 2015 (2003).

D.W. Urry, T. Hugel, M. Seitz, H.E. Gaub, L. Sheiba, J. Dea, J. Xu, L. Hayes, F. Prochazka & T. Parker
Elastomeric Proteins
Ed.: P. R. Shewry, A. S. Tatham, A. J. Bailey, Ideal Protein Elasticity: Elastin Models, Cambridge University Press (2003).


T. Hugel, N.B. Holland, A. Cattani, L. Moroder, M. Seitz & H.E. Gaub
Single-Molecule Optomechanical Cycle
Science, 296 (5570), 1103 (2002).

M. Seitz, T. Hugel & H.E. Gaub
Ein lichtgetriebener Molekülmotor
Spektrum der Wissenschaft, 10, 14 (2002).

M. Seitz, T. Hugel, N.B. Holland & H.E. Gaub
Nanotechnology toward the Organic Photonics
H. Sasabe, GOOTech Ltd. Chitose, Single Molecule Force Spectroscopy by AFM-Related Techniques, Japan (2002)

D.W. Urry, T. Hugel, M. Seitz, H.E. Gaub, L. Sheiba, J. Dea, J. Xu & T. Parker
Elastin: a Representative Ideal Protein Elastomer
Philos Trans R Soc Lond B Biol Sci., 357 (1418), 169 (2002).


T. Hugel & M. Seitz
The Study of Molecular Interactions by AFM Force Spectroscopy
Macromol. Rapid Commun., 22 (13), 989 (2001).<989::AID-MARC989>3.0.CO;2-D

T. Hugel, M. Grosholz, H. Clausen-Schaumann, A. Pfau, H.E. Gaub & M. Seitz
Elasticity of Single Polyelectrolyte Chains and their desorption from solid supports studied by AFM based Single Molecule Force Spectroscopy
Macromolecules, 34 (4), 1039 (2001).


B. Heck, T. Hugel, M. Iijima & G. Strobl
Steps in the formation of the partially crystalline state
Polymer, 41 (25), 8839 (2000).


B. Heck, T. Hugel, M. Iijima, E. Sadiku & G. Strobl
Steps in the transition of an entangled polymer melt to the partially crystalline state
New J. of Phys., 1, 17.1 (1999).

T. Hugel, G. Strobl & R. Thomann
Building lamellae from blocks: The pathway followed in the formation of crystallites of syndiotactic polypropylene
Acta Polymerica, 50 (5-6), 214 (1999).<214::AID-APOL214>3.0.CO;2-I