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A three-color single-molecule FRET approach to studying correlated interactions in proteins

This software kit allows you to analyze three-color smFRET data with a 3D ensemble Hidden Markov Model approach. This maximum likelihood approach yields the full kinetic information and permits the detection of coordinated interactions in complex protein systems.

The software comes with the complete source code, an ascii importer and an example data set for testing.

Please find the full paper and a video tutorial here:


Please enter your email address to download the software tool and sample data (it will be used for update announcements only). By doing so you accept the MIT software license. The software requires Igor Pro 6.




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